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KMID : 0367420050480080881
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Journal of Korean Pediatric Society
2005 Volume.48 No. 8 p.881 ~ p.885
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Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells
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Kwon Byoung-Chul
Kim Eun-Soo
Kim Kyung-Won
Song Tae-Won
Shon Myung-Hyun
Shin Myeong-Heon
Kim Kyu-Earn
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Abstract
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Purpose: Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils.
Methods: Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1.
Results: Hyperosmolar stimuli didn’t induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium.
Conclusion: We found that hyperosmolar stimuli don’t cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
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KEYWORD
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Exercise-induced asthma, Hyperosmolar, EoL-1 cells
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